Test System PCR RT Bluetang-SBC
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Test system for the detection of Bluetongue virus RNA in biological material using reverse transcription and polymerase chain reaction with real-time fluorescent detection.
Purpose
Detection of Bluetongue virus RNA (Bluetongue virus BTV) in clinical and pathological material, as well as in blood-sucking insects, virus carriers using a combined reverse transcription and RNA amplification reaction with real-time fluorescent detection, and for the detection of the host cell's housekeeping gene presented in the sample as an endogenous control of the extraction and amplification stage. Designed for the analysis of 55 samples.
Description and characteristics
Components appear as a colorless, transparent, slightly opalescent liquid.
The kit includes the following components:
Principle of the method
Bluetongue virus RNA is detected by reverse transcription of viral DNA to obtain cDNA and polymerase chain reaction for amplification of a fragment of the resulting cDNA matrix. Both reactions are performed sequentially in a single PCR tube (one-tube), which reduces and simplifies the analysis procedure, reduces the risk of contamination, and the possibility of error when transferring cDNA to another PCR tube.
Amplification products are detected in real-time mode using the principle of cleavage of the fluorescent label at the 5' end of the oligonucleotide probe.
Analytical sensitivity of the kit is no less than 10 genome equivalents per reaction (103,3 GE/cm3).
Production form
Components included in the test system are packaged in polypropylene microtubes with screw caps with a capacity of 2 cm3 and 0.5 cm3.
Storage and transportation conditions
The test system is transported and stored in the manufacturer's packaging in a light-protected place at a temperature of -18 to -24°C. Repeated freezing and thawing of the test system components and transportation at a temperature of 2 to 8°C for up to seven days are allowed.
Shelf life - 15 months
Test system for the detection of Bluetongue virus RNA in biological material using reverse transcription and polymerase chain reaction with real-time fluorescent detection.
Purpose
Detection of Bluetongue virus RNA (Bluetongue virus BTV) in clinical and pathological material, as well as in blood-sucking insects, virus carriers using a combined reverse transcription and RNA amplification reaction with real-time fluorescent detection, and for the detection of the host cell's housekeeping gene presented in the sample as an endogenous control of the extraction and amplification stage. Designed for the analysis of 55 samples.
Description and characteristics
Components appear as a colorless, transparent, slightly opalescent liquid.
The kit includes the following components:
|
№
|
Serial Composition of the test system Amount |
In vial Number of |
Vals Number of |
Determinations |
|
1 |
PCR mixture |
275 µl |
1 |
55 |
|
2 |
Enzyme |
275 µl |
1 |
|
|
3 |
Buffer |
550 µl |
1 |
|
|
4 |
Control - |
100 µl |
1 |
20 |
|
5 |
Control+ |
100 µl |
1 |
Principle of the method
Bluetongue virus RNA is detected by reverse transcription of viral DNA to obtain cDNA and polymerase chain reaction for amplification of a fragment of the resulting cDNA matrix. Both reactions are performed sequentially in a single PCR tube (one-tube), which reduces and simplifies the analysis procedure, reduces the risk of contamination, and the possibility of error when transferring cDNA to another PCR tube.
Amplification products are detected in real-time mode using the principle of cleavage of the fluorescent label at the 5' end of the oligonucleotide probe.
Analytical sensitivity of the kit is no less than 10 genome equivalents per reaction (103,3 GE/cm3).
Production form
Components included in the test system are packaged in polypropylene microtubes with screw caps with a capacity of 2 cm3 and 0.5 cm3.
Storage and transportation conditions
The test system is transported and stored in the manufacturer's packaging in a light-protected place at a temperature of -18 to -24°C. Repeated freezing and thawing of the test system components and transportation at a temperature of 2 to 8°C for up to seven days are allowed.
Shelf life - 15 months