Test System PCR RT Listeriosis-SBC
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Test system for the detection of Listeria monocytogenes bacteria DNA in biological material using polymerase chain reaction with real-time fluorescent detection.
Purpose
Detection of Listeria monocytogenes bacteria DNA in biological material from animals (blood, discharge from the genital organs of an aborting animal, discharge from the nose and eyes, milk, urine, rodent feces). Designed for the analysis of 55 samples.
Description and characteristics
Components appear as a colorless, transparent, slightly opalescent liquid.
The kit includes the following components:
Principle of the method
The test system allows for the specific amplification of a fragment of the Listeria monocytogenes bacteria genome in a multiplex polymerase chain reaction. Fluorescent signal reading is performed directly during PCR. Detection of amplification products is carried out in real-time mode using the principle of fluorescence label cleavage at the 5' end of the oligonucleotide probe. The analytical sensitivity of the kit is not less than 10 genome-equivalents per reaction (103,3 GE/cm3).
Production form
Components included in the test system are packaged in polypropylene microtubes with screw caps with a capacity of 2 cm3 and 0.5 cm3.
Storage and transportation conditions
The test system is transported and stored in the manufacturer's packaging, protected from light at a temperature of -18 to -24°C. Repeated freezing and thawing of test system components and transportation at a temperature of 2 to 8°C for up to seven days are permissible.
Shelf life - 15 months
Test system for the detection of Listeria monocytogenes bacteria DNA in biological material using polymerase chain reaction with real-time fluorescent detection.
Purpose
Detection of Listeria monocytogenes bacteria DNA in biological material from animals (blood, discharge from the genital organs of an aborting animal, discharge from the nose and eyes, milk, urine, rodent feces). Designed for the analysis of 55 samples.
Description and characteristics
Components appear as a colorless, transparent, slightly opalescent liquid.
The kit includes the following components:
|
№
|
Serial Composition of the test system Amount |
In vial Number of |
Vals Number of |
Determinations |
|
1 |
PCR mixture |
275 µl |
1 |
55 |
|
2 |
Enzyme |
275 µl |
1 |
|
|
3 |
Buffer |
550 µl |
1 |
|
|
4 |
Control - |
100 µl |
1 |
20 |
|
5 |
Control+ |
100 µl |
1 |
Principle of the method
The test system allows for the specific amplification of a fragment of the Listeria monocytogenes bacteria genome in a multiplex polymerase chain reaction. Fluorescent signal reading is performed directly during PCR. Detection of amplification products is carried out in real-time mode using the principle of fluorescence label cleavage at the 5' end of the oligonucleotide probe. The analytical sensitivity of the kit is not less than 10 genome-equivalents per reaction (103,3 GE/cm3).
Production form
Components included in the test system are packaged in polypropylene microtubes with screw caps with a capacity of 2 cm3 and 0.5 cm3.
Storage and transportation conditions
The test system is transported and stored in the manufacturer's packaging, protected from light at a temperature of -18 to -24°C. Repeated freezing and thawing of test system components and transportation at a temperature of 2 to 8°C for up to seven days are permissible.
Shelf life - 15 months