Test System PCR RT Leukosis- SBC

INN

Test system for the detection of DNA of the bovine leukosis virus provirus (Bovine leukosis virus, BLV) in biological material by the polymerase chain reaction with fluorescent detection in real-time mode.

Purpose

The detection of DNA of the bovine leukosis virus provirus (Bovine leukosis virus, BLV) in biological material (whole blood) by the DNA amplification method with fluorescent detection in real-time mode and for the identification of the host cell housekeeping gene represented in the sample as an endogenous control of the extraction and amplification stage. Designed for the analysis of 55 samples.

Description and characteristics

Components in appearance are colorless, transparent, slightly opalescent liquid.

The kit includes the following components:

№	Serial Composition of the test system Amount	In vial Number of	Vals Number of	Determinations
1	PCR mixture	275 µl	1	55
2	Enzyme	275 µl	1
3	Buffer	550 µl	1
4	Control -	100 µl	1	20
5	Control+	100 µl	1	20

Principle of the method

The test system allows specific amplification of a fragment of the bovine leukosis virus provirus genome (Bovine leukosis virus, BLV) in a multiplex polymerase chain reaction. The fluorescent signal is read directly during PCR.

The detection of amplification products is carried out in real-time mode using the principle of cleavage of the fluorescent label at the 5’ end of the oligonucleotide probe.

The analytical sensitivity of the kit is not less than 10 genome equivalents per reaction (10^3,3 GE/cm³).

Production form

Components included in the test system are packaged in polypropylene microtubes with screw-on caps with a capacity of 2cm³and 0.5 cm³.

Storage and transportation conditions

The test system is transported and stored in the manufacturer's packaging in a light-protected place at a temperature of -18 to 24°C. Repeated freezing and thawing of the test system components and transportation at a temperature of 2 to 8°C for up to seven days are allowed.

Shelf life - 15 months

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