Test System PCR RT ASF- SBC
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Test system for detecting African swine fever virus DNA in biological material using polymerase chain reaction with real-time fluorescence detection.
Purpose
The test system is designed for detecting African swine fever virus (Pestis africana suum) DNA in clinical material and pathological material from deceased animals using DNA amplification with real-time fluorescence detection and for identifying the host cell domestic gene in the sample as an endogenous control for the extraction and amplification stages. The test system is designed for the analysis of 55 samples.
Description and characteristics
Components appear as a colorless, transparent, slightly opalescent liquid.
The kit includes the following components:
Principle of the method
The test system allows for the specific amplification of a fragment of the African swine fever virus genome in a multiplex polymerase chain reaction. Fluorescent signal reading is carried out directly during PCR.
Amplification products are detected in real-time mode using the principle of cleavage of the fluorescent label at the 5' end of the oligonucleotide probe. Analytical sensitivity of the kit is no less than 10 genome equivalents per reaction (103,3 GE/cm3).
Production form
Components included in the test system are packaged in polypropylene microtubes with screw-on caps with a capacity of 2 cm3 and 0.5 cm3.
Storage and transportation conditions
The test system is transported and stored in the manufacturer's packaging in a light-protected place at a temperature of -18 to 24ºC. Multiple freeze-thaw cycles and transportation at a temperature of 2 to 8°C for up to seven days are permitted.
Shelf life - 15 months.
Test system for detecting African swine fever virus DNA in biological material using polymerase chain reaction with real-time fluorescence detection.
Purpose
The test system is designed for detecting African swine fever virus (Pestis africana suum) DNA in clinical material and pathological material from deceased animals using DNA amplification with real-time fluorescence detection and for identifying the host cell domestic gene in the sample as an endogenous control for the extraction and amplification stages. The test system is designed for the analysis of 55 samples.
Description and characteristics
Components appear as a colorless, transparent, slightly opalescent liquid.
The kit includes the following components:
|
№
|
Serial Composition of the test system Amount |
In vial Number of |
Vals Number of |
Determinations |
|
1 |
PCR mixture |
275 µl |
1 |
55 |
|
2 |
Enzyme |
275 µl |
1 |
|
|
3 |
Buffer |
550 µl |
1 |
|
|
4 |
Control - |
100 µl |
1 |
20 |
|
5 |
Control+ |
100 µl |
1 |
Principle of the method
The test system allows for the specific amplification of a fragment of the African swine fever virus genome in a multiplex polymerase chain reaction. Fluorescent signal reading is carried out directly during PCR.
Amplification products are detected in real-time mode using the principle of cleavage of the fluorescent label at the 5' end of the oligonucleotide probe. Analytical sensitivity of the kit is no less than 10 genome equivalents per reaction (103,3 GE/cm3).
Production form
Components included in the test system are packaged in polypropylene microtubes with screw-on caps with a capacity of 2 cm3 and 0.5 cm3.
Storage and transportation conditions
The test system is transported and stored in the manufacturer's packaging in a light-protected place at a temperature of -18 to 24ºC. Multiple freeze-thaw cycles and transportation at a temperature of 2 to 8°C for up to seven days are permitted.
Shelf life - 15 months.