Test System PCR RT Influenza A - SBC
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Test system for the detection of Influenza A virus RNA in biological material using reverse transcription and polymerase chain reaction with fluorescent detection in real-time mode.
Purpose
Detection of Influenza A virus RNA (Influenza virus A) in clinical and pathological material by the combined reaction of reverse transcription and RNA amplification with fluorescent detection in real-time mode. The test system is designed for the analysis of 55 samples.
Description and characteristics
Components in appearance are colorless, transparent, slightly opalescent liquid.
The kit includes the following components:
Principle of the method
The detection of Influenza A virus RNA is carried out by reverse transcription of viral RNA to obtain cDNA and polymerase chain reaction for amplification of the obtained cDNA matrix fragment. Both reactions are performed sequentially in a single PCR tube (one-tube), which simplifies and shortens the analysis procedure, reduces the risk of contamination, and the possibility of errors when transferring cDNA to another tube for PCR.
Detection of amplification products is performed in real-time using the principle of fluorescence dye release at the 5' end of the oligonucleotide probe.
The analytical sensitivity of the kit is not less than 10 genome equivalents per reaction (103,3 GE/cm3).
Production form
Components included in the test system are packaged in polypropylene microtubes with screw-on caps with a capacity of 2 cm3 and 0.5 cm3.
Storage and transportation conditions
The test system is transported and stored in the manufacturer's packaging in a light-protected place at a temperature of -18 to 24°C. Repeated freezing-thawing of test system components and transportation at a temperature of 2 to 8°C for up to 7 days are allowed.
Shelf life - 15 months
Test system for the detection of Influenza A virus RNA in biological material using reverse transcription and polymerase chain reaction with fluorescent detection in real-time mode.
Purpose
Detection of Influenza A virus RNA (Influenza virus A) in clinical and pathological material by the combined reaction of reverse transcription and RNA amplification with fluorescent detection in real-time mode. The test system is designed for the analysis of 55 samples.
Description and characteristics
Components in appearance are colorless, transparent, slightly opalescent liquid.
The kit includes the following components:
|
№
|
Serial Composition of the test system Amount |
In vial Number of |
Vals Number of |
Determinations |
|
1 |
PCR mixture |
275 µl |
1 |
55 |
|
2 |
Enzyme |
275 µl |
1 |
|
|
3 |
Buffer |
550 µl |
1 |
|
|
4 |
Control - |
100 µl |
1 |
20 |
|
5 |
Control+ |
100 µl |
1 |
Principle of the method
The detection of Influenza A virus RNA is carried out by reverse transcription of viral RNA to obtain cDNA and polymerase chain reaction for amplification of the obtained cDNA matrix fragment. Both reactions are performed sequentially in a single PCR tube (one-tube), which simplifies and shortens the analysis procedure, reduces the risk of contamination, and the possibility of errors when transferring cDNA to another tube for PCR.
Detection of amplification products is performed in real-time using the principle of fluorescence dye release at the 5' end of the oligonucleotide probe.
The analytical sensitivity of the kit is not less than 10 genome equivalents per reaction (103,3 GE/cm3).
Production form
Components included in the test system are packaged in polypropylene microtubes with screw-on caps with a capacity of 2 cm3 and 0.5 cm3.
Storage and transportation conditions
The test system is transported and stored in the manufacturer's packaging in a light-protected place at a temperature of -18 to 24°C. Repeated freezing-thawing of test system components and transportation at a temperature of 2 to 8°C for up to 7 days are allowed.
Shelf life - 15 months